2. Histocytochemistry Series In Situ Hybridization Using Non-radioisotope labeled RNA Probes (4)
نویسندگان
چکیده
منابع مشابه
In situ hybridization of embryos with antisense RNA probes.
Detection of transcripts in situ is a rapid means by which gene expression can be characterized in many systems. In the nematode, Caenorhabditis elegans, the ease with which transgenics can be made and the general reliability of reporter fusion expression patterns, have made this technique comparatively less popular than in other systems. There are, however, still applications in which in situ ...
متن کاملA Simplified In Situ Hybridization Protocol Using Non-radioactively Labeled Probes to Detect Abundant and Rare mRNAs on Tissue Sections
bstract The article describes a simplified and efficient protocol for non-radioactive in situ hybridization experiments on tissue sections. The sensitivity of the method allows the expression of genes to be studied with transcript levels ranging from very low (20–30) to high (several thousands) copy numbers per cell. The immediate procession of cryosections to the ISH protocol after sectioning,...
متن کاملDetection of individual endogenous RNA transcripts in situ using multiple singly labeled probes.
Measurements of gene expression within single cells have revealed startling variability otherwise hidden in bulk measurements. Here, we present an in situ hybridization method capable of detecting individual mRNA molecules, thus permitting the accurate quantification and localization of mRNA within fixed sample. Our in situ protocol involves probing the target mRNA using a series of singly labe...
متن کاملSensitive two-color whole-mount in situ hybridizations using digoxygenin- and dinitrophenol-labeled RNA probes.
Two-color whole-mount in situ hybridization is a powerful technique for comparing the spatial expression patterns of two genes in developing embryos or within a given adult tissue. Many current procedures use two genespecific antisense RNA probes, each incorporating a different chemical moiety that is recognized by a specific antibody. Typically, the first gene-specific RNA probe is labeled wit...
متن کاملIn situ hybridization with oligonucleotide probes.
Equipment and reagents • Glass coverslips, 22x22mm • 6-well plates • 4% freshly made paraformaldehyde (Electron Microscopy Sciences) in PBS, pH 7.4 • Triton X-100 (Sigma) • Parafilm • Forceps, Dumont, GG (Electron Microscopy Sciences) • Avidin-DCS-Texas Red or –fluorescein (Vector) • Filter paper • Mounting medium (Molecular Probes) • Microscopy coverslide • Deionized formamide (Ambion) • Yeast...
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ژورنال
عنوان ژورنال: Nihon Ika Daigaku Igakkai Zasshi
سال: 2010
ISSN: 1349-8975,1880-2877
DOI: 10.1272/manms.6.23